Antibacterial and cytotoxicity effects of biogenic palladium nanoparticles synthesized using fruit extract of Couroupita guianensis Aubl.

Publication date: February 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 1Author(s): Sathishkumar Gnanasekar, Jeyaraj Murugaraj, Balakrishnan Dhivyabharathi, Varunkumar Krishnamoorthy, Pradeep K Jha, Prabukumar Seetharaman, Ravikumar Vilwanathan, Sivaramakrishnan SivaperumalAbstractHerein, we report a facile route to synthesize palladium nanoparticles (CGPdNPs) using the aqueous fruit extract of C. guianensis Aubl. as a potent biological reducing agent. Reduction of PdCl2 solution into their nano scale was confirmed with the formation of a black precipitate which gives a reduced absorbance in UV–vis spectroscopy. Fourier transform infrared spectroscopy (FTIR) reveals the active role of phenolic constituents from C. guianensis in reduction and surface functionalization of nanoparticles (NPs). Dynamic light scattering (DLS) and zeta potential analysis confirms the generation of polydispersed highly stable NPs with large negative zeta value (−17.7 mV). Interestingly, X-ray Diffraction (XRD) pattern shows that the synthesized CGPdNPs were face centered cubic crystalline in nature. The HRTEM micrographs of CGPdNPs displays well-dispersed, spherical NPs in the size ranges between 5 and 15 nm with an average of 6 nm. It was also noticed that the synthesized CGPdNPs possess an effective antimicrobial activity against different bacterial pathogens. On the other hand, in vitro cell viability (MTT) assays reveals that the synthesized CGPdNPs exhibited a...
Source: Journal of Applied Biomedicine - Category: Biotechnology Source Type: research