Genotyping of β-lactams resistant staphylococci isolated from bovine subclinical mastitis

Publication date: Available online 5 June 2018Source: Beni-Suef University Journal of Basic and Applied SciencesAuthor(s): Ahmed H. Abed, Al Sayed R. Attia, Ahmed A. AtiaAbstractStaphylococci are one of the most significant causative mastitis pathogens in both clinical and subclinical cases. The present study aimed to study the prevalence of staphylococcal subclinical mastitis studying their antibiogram and detection of the prevalence mecA and blaZ genes in β-lactams resistant isolates using polymerase chain reaction (PCR). A total of 433 Staphylococcus species were recovered from 493 subclinically mastitic quarters with a prevalence of 87.8%. S. aureus were the most prevalent isolates as 237 isolates (48%). Also, 196 Coagulase-Negative Staphylococci (CNS) isolates (39.8%); representing 8 species, were recovered of which S. xylosus was the most predominant (36.7%), followed by S. epidermidis (14.8%), S. chromogenes (13.3%), S. haemolyticus (11.2%) and S. saprophyticus (9.7%). Results of antimicrobial susceptibility testing showed high resistances against ampicillin and cefoxitin while a moderate resistance was recorded against amoxicillin. PCR was applied on 40 β-lactam resistant Staphylococcus isolates (20 S. aureus and 20 CNS) for detection of mecA and blaZ genes. The two genes were detected in 75% and 65% of S. aureus isolates as well as in 70% and 55% of CNS isolates, respectively.
Source: Beni Suef University Journal of Basic and Applied Sciences - Category: Science Source Type: research