In vivo electroporation of a codon-optimized BERopt DNA vaccine protects mice from pathogenic Mycobacterium tuberculosis aerosol challenge

In this study, we investigated a tuberculosis DNA vaccine BERopt, which contained a codon-optimized fusion immunogen Ag85B-ESAT-6-Rv2660c for enhanced mammalian cell expression and immunogenicity. BERopt immunization through in vivo electroporation in BALB/c mice induced surprisingly high frequencies of Ag85B tetramer+ CD8+ T cells in peripheral blood and IFN- γ-secreting CD8+ T cells in splenocytes.

Source: Tuberculosis - July 9, 2018 Category: Respiratory Medicine Authors: Jiansong Tang, Yi Cai, Jianguo Liang, Zhiwu Tan, Xian Tang, Chi Zhang, Lin Cheng, Jingying Zhou, Haibo Wang, Wing-Cheong Yam, Xinchun Chen, Hui Wang, Zhiwei Chen Source Type: research