Low-template methods yield limited extra information for PowerPlex ® Fusion 6C profiling

Forensic casework samples often contain low amounts of DNA for which standard methods for short tandem repeat (STR) typing may fail to produce a useful DNA profile. As a rule of thumb, we regard the DNA contribution of a donor to a sample low-template (LT) when the allele calls are below the established stochastic threshold. According to this view, a mixed DNA profile can have both high-template and low-template contributors. In these cases, one may consider to sensitize DNA profiling by, for example, using more amplification cycles or higher capillary electrophoresis (CE) injection settings [1 –12].

Source: Legal Medicine - June 1, 2018 Category: Forensic Medicine Authors: Francisca Duijs, Linda van de Merwe, Titia Sijen, Corina C.G. Benschop Source Type: research