Selective multistage extraction process of biomolecules from vine shoots by a combination of biological, chemical, and physical treatments

Publication date: June 2018Source: Comptes Rendus Chimie, Volume 21, Issue 6Author(s): Hiba N. Rajha, Sally El Kantar, Charbel Afif, Nadia Boussetta, Nicolas Louka, Richard G. Maroun, Eugene VorobievAbstractA multistep extraction process was proposed to recover polyphenols, reducing sugars, and soluble lignin from vine shoots. A physical pretreatment by high voltage electrical discharges (HVED) was followed by an enzymatic hydrolysis and a final delignification step by alkaline hydrolysis. HVED before enzymatic hydrolysis enhanced the extraction of polyphenols (+72%), reducing sugars (+43%), and soluble lignin (+104%) as compared to control experiments (enzymatic hydrolysis). HVED also reinforced the subsequent delignification process by reducing 10% lignin content in exhausted residues. Identification and quantification of ferulic acid, resveratrol, p-coumaric acid, and hydroxybenzoic acid were carried out using high-performance liquid chromatography.RésuméUn procédé d'extraction sélectif et multi-étapes a été proposé pour récupérer les polyphénols, les sucres réducteurs et la lignine soluble des sarments de vigne. Un prétraitement physique par les décharges électriques de haute tension (DEHT) a été suivi d'une hydrolyse enzymatique et d'une étape de délignification finale par hydrolyse alcaline. L'application des DEHT avant l'hydrolyse enzymatique améliore l'extraction des polyphénols (+72%), des sucres réducteurs (+43%) et de la lignine soluble (+104...

Source: Comptes Rendus Chimie - July 5, 2018 Category: Chemistry Source Type: research