Characterization and applications of exopolysaccharide produced by marine Bacillus altitudinis MSH2014 from Ras Mohamed, Sinai, Egypt

Publication date: Available online 8 June 2018Source: Egyptian Journal of Basic and Applied SciencesAuthor(s): Sahar S. Mohamed, Shaimaa K. Amer, Manal S. Selim, Hala M. RifaatAbstractThe present study aims to survey exopolysaccharides (EPSs) production in 29 bacterial strains isolated from the sediments around the mangrove trees in Ras Mohamed area, Red Sea Coast, Sinai Peninsula, Egypt. Two of the strains were able to produce EPSs. A higher yield of EPS was obtained from isolate No. 12. Strain identification resulted in a close similarity with Bacillus altitudinis. The produced EPS was characterised as a heteropolysaccharide containing mannouronic acid, glucose, and sulphate. A gel permeation chromatography was used to estimate the EPS molecular weight which found to be 4.23 × 105 Dalton. The typical pattern of polysaccharide absorbance was supported by the infrared spectrum. The EPS appears significant in vitro antitumor activities against two cancers cells EACC and lung cancer A-549. Furthermore, a broad range of bacteria and fungi were inhibited with purified EPS.
Source: Egyptian Journal of Basic and Applied Sciences - Category: Science Source Type: research