Biochemical and Apoptotic Biomarkers of Experimentally Induced Traumatic Brain Injury: In Relation to Time since Death

Publication date: Available online 31 January 2018Source: Beni-Suef University Journal of Basic and Applied SciencesAuthor(s): Amany Abdel-Rahman Mohamed, Khlood M. Elbohi, Nabela I. El Sharkawy, Mona A. HassanAbstractThe present study declares the relationship between the cause of death and postmortem biochemical marker alterations in CSF, serum and plasma. In addition, immunohistochemical and microarchitecture examination of brain tissue of White New Zealand rabbits at different PMI after traumatized death. Thirty adult male White New Zealand rabbits were divided into two main equal groups; the first was physically killed by cervical dislocation and the second through head trauma. Each group was subdivided into three PMIs (zero, 6, and 12hrs PM). CSF was used to detect the levels of K+, Na+, Ca++, albumin While, lactic acid, hypoxanthine, ammonia and uric acid concentrations were measured in plasma. Estimation of HMGB1, IL-1β and TNF-α were assessed in serum. In addition to immunohistochemical observations of Bcl-2 and P53 apoptotic proteins in brain tissue. The results revealed that some of the examined parameters as K+, Na+, albumin, ammonia, hypoxanthine and HMGB1 had the potential role in estimation of PMI at examined time periods in physical and traumatized death. Traumatic death induced severe cerebral hemorrhages and necrosis of cerebral parenchyma than physical death. Immunohistochemical results of P53 and Bcl-2 in brain tissue declared focal positive reactions of...
Source: Beni Suef University Journal of Basic and Applied Sciences - Category: Science Source Type: research