Recombinant Expression and Purification of Mouse Nectin-like 4 Glycoprotein in 293ET Cell Line

ConclusionsBy using modified AP mammalian protein expression system, we can easily screen the high productive stable cell lines by using AP activity assay. By adding mannosidase inhibitor kifunensine into the medium and cutting purified protein by using endoglycosidase H, we can obtain deglycosylated Necl-4 protein in milligram quantities. Our method might throw a light on the expression and purification of glycoprotein for structural and functional studies.
Source: Chinese Medical Sciences Journal - Category: General Medicine Source Type: research