A synthetic biological secondary metabolite, Lycogen™, produced and extracted from Rhodobacter sphaeroides WL-APD911 in an optimizatioal scale-up strategy

Publication date: December 2017Source: Food Science and Human Wellness, Volume 6, Issue 4Author(s): Cheng-Chin Wang, Shi-Ying Huang, Shu-Hung Huang, Zhi-Hong Wen, Jyun-Ying Huang, Wen-Sheng Liu, Hui-Min David WangAbstractThe optimization of fermentation medium is important for synthetic biological secondary metabolite productions. The effect of rotation speed, inoculum amount, and medium supplements on the cell growth and Lycogen™ secretion of photobacterium Rhodobacter sphaeroides WL-APD911 was evaluated. The results reveal that a higher rotational speed exhibit a higher cell density, and the increasing in the amount of inoculum amount show a slight augment on the growth of R. sphaeroides WL-APD911.In the case of nitrogen sources adding, Lycogen™ production was achieved with a 0.5 mM l-lysine supplementation. Moreover, the attention of Tween 80 presented a tremendous increase in the secondary metabolite. Response surface methodology (RSM) exhibited the optimization of medium supplements for Lycogen™ invention is accomplished at molasses concentration of 10 g/L, yeast extract concentration of 40 g/L, 0.3% Tween 80 and NaCl concentration of 5 g/L, respectively. Further, the batch fermentation is carried out in both 5 L and 20 L fermentors to study the scale-up process factors to be adopted. At a 20 L fermentor, Lycogen™ yields under the optimal culture condition are over 2 times than in the shake flask. The present results provide the...

Source: Food Science and Human Wellness - July 5, 2018 Category: Food Science Source Type: research