Antibacterial activity of green silver nanoparticles synthesized from Anogeissus acuminata against multidrug resistant urinary tract infecting bacteria in vitro and host-toxicity testing

Publication date: May 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 2Author(s): Monali P. Mishra, Rabindra N. PadhyAbstractSilver nanoparticles (AgNPs) with aqueous leaf-extract of the timber-yielding plant Anogeissus acuminata were synthesized for in vitro control of pathogenic bacteria. Characterization of AgNPs with ultraviolet-visible spectroscopy, scanning electron microscopy (SEM), energy dispersive X-ray (EDX) spectroscopy, X-Ray diffraction (XRD) study and Fourier transformed infrared spectroscopy (FTIR) study was done for a confirmation of the synthesis. The SEM analysis confirmed that the metal particles were below 100 nm size. The antibacterial activity of AgNPs was monitored by agar-well diffusion method against 11 multidrug resistant (MDR) urinary tract infection (UTI) causing pathogenic bacteria, isolated from clinical samples. At 15 μg/ml AgNPs, values of the zone of inhibition (ZI) ranged from 19 to 13 mm, while against the standard antibiotic, gentamicin 30 μg/ml ZI ranged from 28 to 20 mm. Host toxicity testing of AgNPs with cultured lymphocytes from human umbilical cord blood in vitro was done; at 3000 mg/l AgNPs, 25% of cell death occurred. Thus, the synthesized AgNPs with aqueous leaf extract of A. acuminata could control most MDR UTI bacteria without any toxicity to human lymphocytes.Graphical abstract
Source: Journal of Applied Biomedicine - Category: Biotechnology Source Type: research