Integrated Transcriptomic and Epigenomic Analysis of Ovarian Cancer Reveals Epigenetically Silenced GULP1

Many epigenetically inactivated genes involved in ovarian cancer (OC) development and progression remain to be identified. Therefore, in this study we undertook an integrated approach that consisted of identification of genome-wide expression patterns of primary OC samples and normal ovarian surface epithelium along with pharmacologic unmasking strategy using 3 OC and 3 immortalized normal ovarian epithelial cell lines. Our filtering scheme identified 43 OC specific methylated genes and among the 5 top candidates (GULP1, CLIP4, BAMBI, NT5E, TGFB2), we performed extended studies of GULP1.

Source: Cancer Letters - July 2, 2018 Category: Cancer & Oncology Authors: Leonel Maldonado, Mariana Brait, Evgeny Izumchenko, Shahnaz Begum, Aditi Chatterjee, Tanusree Sen, Myriam Loyo, Alvaro Barbosa, Maria Luana Poeta, Eugene Makarev, Alex Zhavoronkov, Vito M. Fazio, Roberto Angioli, Carla Rabitti, Mate Ongenaert, Wim Van Cri Tags: Original Articles Source Type: research