DNA polymerase eta is regulated by poly(rC)-binding protein 1 via mRNA stability

In this study, we found that the levels of both POLH transcript and protein were decreased upon knockdown of the transcript encoding poly(rC)-binding protein 1 (PCBP1). We also found that the half-life of POLH mRNA was markedly decreased upon knockdown of PCBP1. Moreover, we found that PCBP1 directly bound to POLH 3'UTR and the PCBP1-binding site in POLH mRNA is an atypical AU-rich element. Finally, we showed that the AU-rich element in POLH 3'UTR was responsive to PCBP1 and sufficient for PCBP1 to regulate POLH expression. Altogether, we uncovered a novel mechanism by which POLH expression is controlled by PCBP1 via mRNA stability.

Source: BJ Gene - September 30, 2014 Category: Biochemistry Authors: C Ren, S Cho, Y Jung, X Chen Tags: BJ Gene Source Type: research