Inducing Lipid Reorganization and the Acrosome Reaction in Stallion Spermatozoa

Conditions for successful in vitro capacitation of equine spermatozoa are not established. In other species, increased plasma membrane fluidity, externalization of phosphatidylethanolamine (PE) and phosphatidylserine, and cholesterol efflux in response to bicarbonate (HCO3-) are considered to indicate capacitation and preparation of spermatozoa for the acrosome reaction. We investigated whether extracellular bicarbonate (bic), bovine serum albumin (BSA) and calcium (Ca2+) modulated membrane fluidity, PE exposure and acrosome integrity in equine spermatozoa.

Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: R. Hoogendijk, B.M. Gadella, T.A.E. Stout, H.H.W. Henning Source Type: research