The Effects of Extender Glucose Concentration and Storage Temperature on Stallion Semen Quality following Cooled Storage

Glucose is a common metabolic substrate in extenders used for cooled storage; yet, the optimal concentration of glucose is unknown, and interaction of glucose concentration and storage temperature has not been investigated. One ejaculate from each of 7 stallions was collected and diluted (1:1, v/v) with a milk-based extender at 4 different glucose concentrations: 0, 67, 147, or 270 mM (G0, G67, G147, and G270). Extended semen was subjected to cushioned centrifugation, and sperm were resuspended to 30 x 106/mL with the same extender containing 10% (v/v) of fresh, homologous seminal plasma.
Source: Journal of Equine Veterinary Science - Category: Veterinary Research Authors: Source Type: research