Filtration of Commercial Extenders Prior to Refreezing Stallion Sperm for ICSI

Intracytoplasmic sperm injection (ICSI) is a feasible technique for in vitro embryo production and optimization of stallion sperm utilization. The efficient use of frozen-thawed sperm can be improved by refreezing [1], which is an option for stallion sperm when availability is limited [2] and a method that has been successful in other species, including human and bovine [3,4]. Stallion sperm can be cryopreserved using extenders that contain skim milk, egg yolk, and glycerol as cryoprotectants [5].

Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: G.D. Catandi, R.A. Gonzalez-Castro, J.M. Trentin, M.L. Corder, F.A. Sanches, J.E. Stokes, J.K. Graham, E.M. Carnevale Source Type: research