Effect of Sperm Concentration of the Frozen Ejaculate of Donkeys on Post-thaw Semen Quality

In order to improve sperm bank efficiency and the current protocols for freezing semen in an endangered species such as the Andalusian donkey, we aimed to study the effect of different sperm concentrations in straws during freezing on the quality of frozen-thawed semen. Ejaculates from 6 donkeys of proven fertility (4-15 years old) were used for this study. A total of two ejaculates per stallion were frozen according to standard protocol: initial dilution of semen in extender (INRA 96, IMV, France) at 50x106/ml, the semen was split between 6 conical tubes and centrifuged (600 × g) for 10 min at room temperature.
Source: Journal of Equine Veterinary Science - Category: Veterinary Research Authors: Source Type: research