Vitrifying Equine Oocytes at the Germinal Vesicle Stage Disturbs Spindle Morphology and Chromosome Alignment

Cryopreserving horse oocytes compromises the ability of immature oocytes to complete meiosis, and reduces the post-fertilization developmental competence of mature oocytes. Previous studies suggest that this reduced competence is, at least in part, related to a compromised ability to form a normal microtubular spindle and complete the meiotic divisions. To better understand the effects of cryopreservation on microtubule spindle function, we examined the effects of vitrifying germinal vesicle stage oocytes on meiotic spindle organization and chromosome alignment after maturation in vitro.
Source: Journal of Equine Veterinary Science - Category: Veterinary Research Authors: Source Type: research