The effect of Glucose Concentration (5.6 mM vs. 17 mM) During IVM on Developmental and Metabolic Markers of Equine Cumulus-oocyte Complex Function

Both M199 and DMEM/F-12 support in vitro maturation (IVM) of equine oocytes and production of viable embryos after ICSI. However, these media differ markedly in glucose concentration: 5.6 mM (M199) vs. 17 mM (DMEM/F-12). Given the known impact of embryonic nutritional programming on fetal and adult development, we evaluated the effect of glucose concentration during IVM on equine cumulus-oocyte complex (COC) function. Abattoir-derived COCs were held overnight, then cultured for 30 h either in individual 10- μl droplets within a petri dish (Study 1) or directly in a Seahorse XFp Bioanalyser plate (Study 2) in M199 with 10% FBS and 5 mU/ml FSH, with 5.6 or 17 mM glucose.
Source: Journal of Equine Veterinary Science - Category: Veterinary Research Authors: Source Type: research