Establishing Monolayers of Differentiated Equine Oviduct Epithelial Cells

Conventional in vitro fertilization with equine gametes is not successful. Since fertilization in vivo takes place in the oviductal ampulla, establishing an in vitro oviduct culture system that mimics the in vivo microenvironment might help us define factors involved in sperm activation and fertilization. In a first step towards developing an in vitro oviduct model, monolayers of differentiated equine oviduct epithelial cells (OECs) were established. Oviducts were obtained from slaughtered adult mares (n=11) at random stages of the estrous cycle.
Source: Journal of Equine Veterinary Science - Category: Veterinary Research Authors: Source Type: research