Effect of Meiotic Stage and Warming Medium Equilibration Atmosphere on Equine Oocyte Vitrification

The objectives of this study were to evaluate a commercial human oocyte vitrification method (Cryotech ®) for vitrification of immature and in vitro-matured equine oocytes, with and without cumulus cells, and to evaluate the effect of warming medium equilibration atmosphere on oocyte developmental competence. Cumulus-oocyte complexes (COCs) were obtained from slaughterhouse-derived ovaries. The COCs (n = 551) were held overnight at room temperature, pipetted to ∼4 layers of cumulus cells, then divided into immature (Imm) or in vitro matured (Mat) groups.
Source: Journal of Equine Veterinary Science - Category: Veterinary Research Authors: Source Type: research