The Effect of Pre- and Post-injection Procedural Factors on Blastocyst Rate After ICSI

Use of intracytoplasmic sperm injection (ICSI) is increasing in the equine industry, but little information is available on factors affecting embryo production in this system. Often, clinical ICSI is performed in multiple mares using the same semen, of variable quality. Swim-up separation (SU) is thought to select for higher-quality sperm. If insufficient sperm are available in the first preparation, it is unclear if remaining thawed semen can be utilized for a second preparation, as prolonged exposure to components of freezing extender may affect sperm viability.
Source: Journal of Equine Veterinary Science - Category: Veterinary Research Authors: Source Type: research