Meiotic Spindle Characteristics of Oocytes After Vitrification at Germinal Vesicle Stage, Warming and Maturation In Vitro and Developmental Potential After ICSI

The oocyte cytoskeleton can be damaged during cryopreservation, potentially compromising normal embryonic development. Aims of this study were to investigate the effect of vitrification of immature (germinal vesicle, GV) oocytes on spindle integrity and embryo development after intracytoplasmic sperm injection (ICSI). Immature oocytes were collected from excised ovaries or live mares, 3-16 yr. Oocytes were assigned to Control (nonvitrified) or Vitrified groups. Oocytes were vitrified as previously described (Kuwayama et al., Reproductive BioMedicine Online 2005) using the Cryotop method and a commercial medium.
Source: Journal of Equine Veterinary Science - Category: Veterinary Research Authors: Source Type: research