Aspiration of Yolk Sac Fluid from the Early Pregnant Mare for Fetal Genotyping

Equine fetal genotyping has multiple clinical applications but currently can be accomplished only via embryo biopsy, which requires embryo recovery, micromanipulation, and transfer. We investigated the possibility of obtaining fluid from the conceptus for fetal genotyping via transvaginal ultrasound-guided aspiration in pregnant mares. Genotyping and assessment of maternal contamination was performed by analyzing histograms for 17 microsatellite ID markers. Areas under the curves were compared for each allele in recovered fluid vs.
Source: Journal of Equine Veterinary Science - Category: Veterinary Research Authors: Source Type: research