The Cys78-Asn88 loop region of the Campylobacter jejuni CstII is essential for {alpha}2,3-sialyltransferase activity: analysis of the His85 mutants

In this study, the role of His85 in the activity of CstII has been investigated by mutating it to Ala, Phe, Trp or Tyr. His85 is found to be essential for α2,3-sialyltransferase activity but not α2,8-sialyltransferase activity. Although no gross changes are observed in secondary and tertiary structures, thermal stability is affected by His85 mutation. MD simulations show changes in the flexibility of the loop regions including those in the binding site.
Source: Journal of Biochemistry - Category: Biochemistry Authors: Tags: Regular Papers Source Type: research