CD39 and CD73 in the aortic valve – biochemical and immunohistochemical analysis in valve cell populations and its changes in valve mineralization

The calcific aortic valve disease (CAVD) is a common heart pathology that involves inflammation, fibrosis and calcification of aortic valve leaflets. All these processes could be affected by changes in the extracellular purinergic signaling that depend on the activity of ecto-nucleotidases, mainly ecto-nucleoside triphosphate diphosphohydrolase 1 (CD39, eNTPD1) and ecto-5 ’nucleotidase (CD73, e5NT).Objective and Methods.We investigated the localization of CD39 and CD73 proteins in human non-calcified and calcified aortic valves using immunohistochemistry together with analysis of NTPDases and e5NT activities in aortic valve homogenates by analysis of substrate into product conversion by high-performance liquid chromatography (HPLC).

Source: Cardiovascular Pathology - June 7, 2018 Category: Cardiology Authors: Ewa Kaniewska-Bednarczuk, Barbara Kutryb-Zajac, Padmini Sarathchandra, Iwona Pelikant-Malecka, Alicja Sielicka, Izabela Piotrowska, Ewa M. Slomi ńska, Adrian H. Chester, Magdi H. Yacoub, Ryszard T. Smolenski Source Type: research