A New Dual-Targeting Real Time RT-PCR Assay for Hepatitis D Virus RNA Detection

In this study, a real-time reverse transcription-polymerase chain reaction (real time RT-PCR) assay targeting two genetic segments was established to detect HDV RNA. Utilizing the WHO International Standard for Hepatitis D Virus RNA, the lower limit of detection was 575 IU/ml, and the linearity of quantification ranged from 575000 IU/ml to 575 IU/ml. 384 HBsAg-positive samples collected from China were tested by this method and HDV antibody detection. Eleven samples were positive for anti-HDV IgG which may persist after HDV resolution, six samples were HDV RNA positive and five samples were positive for anti-HDV IgM.
Source: Diagnostic Microbiology and Infectious Disease - Category: Microbiology Authors: Source Type: research