Roles of p53, NF- κB and the androgen receptor in controlling NGAL expression in prostate cancer cell lines

Publication date: Available online 18 May 2018 Source:Advances in Biological Regulation Author(s): William H. Chappell, Saverio Candido, Stephen L. Abrams, Suzanne Russo, Roger Ove, Alberto M. Martelli, Lucio Cocco, Giulia Ramazzotti, Melchiorre Cervello, Giuseppe Montalto, Linda S. Steelman, Xiaohong Leng, Ralph B. Arlinghaus, Massimo Libra, James A. McCubrey Neutrophil gelatinase-associated lipocalin (NGAL a.k.a lipocalin 2, lnc2) is a secreted protein which can form a complex with matrix metalloproteinase-9 (MMP9). This MMP9/NGAL complex has been associated with metastasis. MMP9 and NGAL are detected in the urine of patients afflicted with many different types of cancer, including prostate cancer. The effects of p53, NF-κB and the androgen receptor (AR) on the expression of NGAL was examined in four prostate cancer cell lines. Prostate cancer cell lines that are AR negative and expressed either mutant or no p53 (DU145 and PC3) displayed higher levels of NGAL expression compared to the prostate cancer cell lines (LNCaP and 22Rv-1) which are AR positive and express wild type (WT) p53. Introduction of WT-p53 into the PC3 prostate cancer cell line, resulted in reduction of the levels of NGAL expression. Conversely, introduction of dominant negative (DN) p53 or a retroviral construct expressing NF-κB into LNCaP cells increased NGAL expression. NGAL expression had functional effects on the ability of the cells to form colonies in soft agar. Whereas suppression...
Source: Advances in Biological Regulation - Category: Biology Source Type: research