Chapter Ten The Structure/Function Relationship in Antimicrobial Peptides: What Can we Obtain From Structural Data?

Publication date: 2018 Source:Advances in Protein Chemistry and Structural Biology, Volume 112 Author(s): Marlon H. Cardoso, Karen G.N. Oshiro, Samilla B. Rezende, Elizabete S. Cândido, Octávio L. Franco Antimicrobial peptides (AMPs) have been widely isolated from most organisms in nature. This class of antimicrobials may undergo changes in their sequence for improved physicochemical properties, including charge, hydrophobicity, and hydrophobic moment. It is known that such properties may be directly associated with AMPs’ structural arrangements and, consequently, could interfere in their modes of action against microorganisms. In this scenario, biophysical methodologies, such as nuclear magnetic resonance spectroscopy, X-ray crystallography, and cryo-electron microscopy, allied to in silico approaches, including molecular modeling, docking, and dynamics nowadays represent an enormous first step for the structural elucidation of AMPs, leading to further structure–function annotation. In this context, this chapter will focus on the main atomic-level experimental and computational tools used for the structural elucidation of AMPs that have assisted in the investigation of their functions.
Source: Advances in Protein Chemistry and Structural Biology - Category: Biochemistry Source Type: research