Direct genotyping of Mycobacterium tuberculosis from Xpert ® MTB/RIF remnants

Genotyping of Mycobacterium tuberculosis (MTB) isolates has markedly improved our knowledge of its transmission dynamics. MIRU-VNTR is considered the reference molecular tool for MTB fingerprinting. However, the dependence of this technique on cultured isolates means that we lack molecular epidemiology data from many settings where culture facilities have not been implemented. Efforts have been made to adapt the MIRU-VNTR procedure to direct analysis of clinical specimens, although implementation of these efforts has not proven successful.

Source: Tuberculosis - May 15, 2018 Category: Respiratory Medicine Authors: Edson T. Mambuque, Estefan ía Abascal, Rouxjeane Venter, Helder Bulo, Emilio Bouza, Grant Theron, Alberto L. García-Basteiro, Darío García-de-Viedma Source Type: research