Lipopeptides are signaled by Toll-like receptor 1, 2 and 6 in endolysosomes

In this study, we established mAbs to TLR1, 2 and 6. TLR1, 2 and 6 were expressed on the surface of B cells, monocytes and dendritic cells in a manner dependent on a TLR-specific chaperone PRAT4A (protein associated with TLR4 A). Cell surface localization of TLR1 or TLR6 was not necessarily required for TLR2 response. Furthermore, a dynamin inhibitor ‘Dynasore’ abolished the lipopeptide responses by preventing lipopeptide internalization into LAMP-1 and LAMP-2 positive compartments. Our findings suggest that lipopeptides elicit TLR1/2 and TLR2/6 signaling in the endolysosomes, but not on the cell surface.

Source: International Immunology - September 20, 2014 Category: Allergy & Immunology Authors: Motoi, Y., Shibata, T., Takahashi, K., Kanno, A., Murakami, Y., Li, X., Kasahara, T., Miyake, K. Tags: Original Research Source Type: research