Serum and Urine Thioflavin-T-Enhanced Fluorescence in Severe PreeclampsiaNovelty and Significance [Preeclampsia]

Common features of amyloid-like proteotoxic aggregates are the ability to bind Congo red (congophilia) and to induce fluorescence of thioflavin-T (ThT). Based on the prior discovery that women with preeclampsia exhibit urine congophilia, we proposed that amyloid-like protein aggregates present in urine also circulate in the bloodstream and this feature is linked to disease severity and clinical phenotype. ThT fluorescence was investigated in 217 paired serum and urine samples from women with severe features of preeclampsia (n=101; median [interquartile range] gestational age [GA], 32 [29–35] weeks), mild features of preeclampsia (n=22; GA, 36 [36–37] weeks), chronic hypertension (n=15; GA, 38 [37–39] weeks), healthy pregnant controls (n=57; GA, 39 [38–39] weeks), and nonpregnant controls (n=22). Serum and urine fluorescence attributable to advanced glycation end products was measured in the same samples with correction for autofluorescence. There were no GA-related changes in ThT fluorescence, although near-term serum ThT fluorescence increased compared with nonpregnant state. Compared with healthy pregnant controls, serum and urine ThT fluorescence was increased in severe features of preeclampsia (P
Source: Hypertension - Category: Cardiology Authors: Tags: Pathophysiology, Hypertension, Preeclampsia Original Articles Source Type: research