Plasmid-Based shRNA Lentiviral Particle Production for RNAi Applications

In this report, we focus exclusively on this step and compare the overall impact of the commercial transfection reagent FuGENE 6 with FuGENE HD. We found that FuGENE HD resulted in at least 5-fold improvement in viral particle titer as assessed by the p24 standard enzyme-linked immunosorbent assay. We present the complete optimized workflow and demonstrate this utility in which a single modification of this transfection step improved the lentiviral particle production.

Source: Journal of Biomolecular Screening - September 19, 2014 Category: Molecular Biology Authors: Shum, D., Djaballah, H. Tags: Technical Note Source Type: research