Gestational Diabetes reduced Sertoli cells in 12 weeks age rat offsprings testis

This study was done to evaluate the effect of induced gestational diabetes on seminiferous tubule of 12 weeks age offspring rats. Materials and Methods 10 Wistar rats' dams were randomly allocated to control and diabetic groups. Streptozotocin was used to induce diabetes in female rats. Dams in diabetic group received 40 mg/kg/BW of streptozotocin at the first day of gestation and control group animals received an equivalent volume of normal saline by intraperitoneally. Six offspring of each group were randomly selected on day 84 postnatal. Five micrometer sections were taken from testes, stained with hematoxylin and eosin. Photographs of sections were taken using Olympus BX51 microscope and a digital camera DP12. Density and number of spermatogenesis cells, leydig cells, sertoli cells, seminiferous tubule diameter and Seminiferous epithelial height and dUTP end-labeling positive cells were evaluated in 50000 μm2 area of seminiferous tubules by Olysia Autobioreport software. Results Spermatogenesis and leydig cells in gestational diabetic offsprings non-significantly reduced in compare to controls. Sertoli cells significantly reduced in gestational diabetic offspring compared to controls. Seminiferous tubular diameter and seminiferous epithelial height non-significantly reduced in gestational diabetic offspring compared to controls. The apoptotic cells in diabetic group non-significantly increased in comparison with controls. The histopathological alterations were not se...
Source: Journal of the Anatomical Society of India - Category: Anatomy Source Type: research