Evaluation of symptomatic patients with resistant discharge

The aim of this study was to detect the presence ofChlamydia trachomatis, Neisseria (N.) gonorrhoeae, Mycoplasma (M.) hominis, M. genitalium, Ureaplasma (U.) urealyticum, andTrichomonas (T.) vaginalis in patients with resistant discharge. The study also evaluated the concordance of the diagnostic tests. Samples from 156 patients were tested by direct microscopy and culture forT. vaginalis and Mycoplasma IES forM. hominis andU. urealyticum.Multiplex Polymerase Chain Reaction (PCR) was used to determine the presence of six agents. Statistical analyses were performed using the SPSS program. Out of 156 patients, 38 had positive result for the agents tested. Of these 38 patients, 28 (73.7%) had single agent positivity and 10 (26.3%) had multiple agent positivity. The detection rate ofU. urealyticum, M. hominis, N. gonorrhoeae, C. trachomatis, T. vaginalis, M. genitaliumspecifically was 10.3%, 9.6%, 6.4%, 3.2%, 2.6%, 0.6% respectively.N. gonorrhoeae andU. urealyticum were the most common in male patients, whileM. hominis andU. urealyticum were mostly found in female patients. Different methods used for detectingT. vaginalis were compared to find that interrater reliability was perfect for culture-direct microscopy ( κ:0.85;P<0.001) and also for culture-PCR ( κ:0.89;P<0.001). The interrater reliability was moderate ( κ:0.53;P<0.001) for PCR-Mycoplasma IES test forM. hominis and fair ( κ:0.21;P<0.007) forU. urealyticum. U. urealyticum andM. hominis were among the most...
Source: Acta Dermatovenerologica Croatica - Category: Dermatology Source Type: research