GH prevents adipogenic differentiation of mesenchymal stromal stem cells derived from human trabecular bone via canonical Wnt signaling
The imbalance between osteogenesis and adipogenesis, which naturally accompanies bone marrow senescence, may contribute to the development of bone-associated diseases, like osteoporosis. In the present study, using primary human mesenchymal stromal cells (hMSCs) isolated from trabecular bone, we assessed the possible effect of GH on hMSC differentiation potential into adipocytes. GH (5 ng/ml) significantly inhibited the lipid accumulation in hMSCs cultured for 14 days in lipogenic medium. GH decreased the expression of the adipogenic genes, CCAAT/enhancer-binding protein alpha (C/EBPα) and adiponectin (ADN) as well as the expression of two lipogenesis-related enzymes, lipopr otein lipase (LPL) and acethylCoA carboxylase (ACACA).
Source: Bone - Category: Orthopaedics Authors: Simona Bolamperti, Michela Signo, Alice Spinello, GianLuigi Moro, Gianfranco Fraschini, Francesca Guidobono, Alessandro Rubinacci, Isabella Villa Tags: Full Length Article Source Type: research