Identification of Leishmania (Viannia) species and clinical isolates of Leishmania (Leishmania) amazonensis from Brazil using PCR-RFLP of the heat-shock protein 70 gene reveals some unexpected observations

In this study, we used a previously described hsp70 PCR-RFLP method for identifying Brazilian Leishmania isolates. We identified two distinct L. (L.) amazonensis hsp70 alleles that resulted in two different RFLP patterns. Also, we found RFLP polymorphisms amongst L. (Viannia) naiffi strains. The profiles of both L. (V.) shawi and L.

Source: Diagnostic Microbiology and Infectious Disease - March 12, 2018 Category: Microbiology Authors: Caroline R. Espada, Paola A. Ortiz, Jeffrey J. Shaw, Aldina M.P. Barral, Jackson M.L. Costa, Silvia R.B. Uliana, Adriano C. Coelho Source Type: research