Rapid Real-Time PCR for the Detection of IMP, NDM, VIM, KPC and OXA-48 Carbapenemase Genes in Isolates and Spiked Stool Samples

An in-house real-time PCR was developed to detect the carbapenemase genes IMP, NDM, VIM, KPC and OXA-48 from both bacterial colonies and directly from fecal material. The assay is a multiplex PCR performed in two tubes with NDM, VIM and IMP genes detected in one tube and OXA-48 and KPC genes in the other. Despite the large amount of primers and probes necessary to cover all variants of especially the VIM and IMP genes, the efficiency of the PCR reactions was from 95 to 100%. When tested on 170 clinical strains and compared to culture results from a reference laboratory, 100% correspondence was found.
Source: Diagnostic Microbiology and Infectious Disease - Category: Microbiology Authors: Source Type: research