A preliminary assessment of the effect of PreCR ™ DNA repair treatment on mixture ratios in two person mixtures

In this study, DNA extracted from known buccal samples was combined into two component mixture samples. These were subjected to UV exposure prior to their amplification with the Promega PowerPlex ® 16HS amplification kit, and subsequent capillary electrophoresis on the ABI 3130xl instrument. Damaged samples were subjected to enzymatic repair treatment and retested to assess the amount of repair. Data showed that there is fidelity associated with the application with profile concordance afte r its use, and a corresponding increase in the amount of recovered alleles post damage.
Source: Science and Justice - Category: Forensic Medicine Authors: Source Type: research