Techniques: In a split sequence

Nature Reviews Neuroscience 19, 254 (2018). doi:10.1038/nrn.2018.37 Author: Sian Lewis Currently available methods for transcriptome sequencing such as single-cell RNA-seq (scRNA-seq) are complex to perform. Here, the authors developed split-pool ligation-based transcriptome sequencing (SPLiT-seq), which involves four rounds of combinatorial barcoding of cellular RNA. The technique involves a series of pipetting steps and requires no
Source: Nature Reviews Neuroscience - Category: Neuroscience Authors: Tags: Research Highlight Source Type: research