Characterization of clostridium difficile isolated from diarrheal patients in a tertiary-care hospital, karnataka, south india.

CHARACTERIZATION OF CLOSTRIDIUM DIFFICILE ISOLATED FROM DIARRHEAL PATIENTS IN A TERTIARY-CARE HOSPITAL, KARNATAKA, SOUTH INDIA. Southeast Asian J Trop Med Public Health. 2016 Nov;47(6):1221-30 Authors: Rituparna C, Mamatha B, Mukhyaprana PM, Manjunatha HH, Gururaja PP, Thandavarayan R Abstract Increase in Clostridium difficile infection in tertiary-care hospitals in Karnataka, South India with a paucity of data on antibiotic susceptibility and genetic characteristics of the pathogen from this region of the country necessitated this study. From April 2012 to December 2014, 480 hospitalized antibiotic-associated diarrhea cases with a history of antibiotic treatment in the previous three weeks were enrolled. Sixteen percent of the samples were positive for C. difficile toxins A and B by rapid enzyme immunoassay, anaerobic culture and multiplex PCR. In 40 representative strains, minimum inhibitory concentrations (MICs) determined by E-test revealed that 39 strains were resistant to imipenem and moxifloxacin (MIC > 32 μg/ml), 38 to clindamycin (MIC > 256 μg/ml) and 19 to tetracycline (MIC > 4 μg/ml), while all 40 strains were susceptible to ampicillin (MIC < 2 μg/ml), ampicillin sulbactam (MIC < 8 μg/ml), metronidazole (MIC < 8 μg/ml) and vancomycin group (MIC < 2 μg/ml). Pulsed field gel-electrophoresis (PFGE) of 13 representative strains grouped them into three clusters: cluster A consisting of two strains ha...
Source: Southeast Asian Journal of Tropical Medicine and Public Health - Category: Tropical Medicine Tags: Southeast Asian J Trop Med Public Health Source Type: research