Detection of helicobacter pylori and virulence-associated genes in saliva samples of asymptomatic persons in northeast thailand.

DETECTION OF HELICOBACTER PYLORI AND VIRULENCE-ASSOCIATED GENES IN SALIVA SAMPLES OF ASYMPTOMATIC PERSONS IN NORTHEAST THAILAND.
Southeast Asian J Trop Med Public Health. 2016 Nov;47(6):1246-56
Authors: Tirapattanun A, Namwat W, Kanthawong S, Wongboot W, Wongwajana S, Wongphutorn P, Chomvarin C
Abstract
The aims of the study were to develop nested-PCR (targeting vacA and
cagA), SYBR green quantitative PCR (targeting 16S rDNA) tests and compared
them with indirect fluorescent-monoclonal antibody (IFA) method for determination
of the prevalence of Helicobacter pylori in 118 saliva samples from asymptomatic
individuals in Khon Kaen, Thailand. Detection limit of both PCR-based assays
was one cell. Prevalence of H. pylori in saliva samples was 55% based on the criterion
of positivity of IFA test and one of the PCR-based methods or positivity of
both PCR assays. Forty-nine percent of H. pylori detected carried cagA, encoding
a cytotoxin associated with severe clinical outcomes. These results imply that the
mouth may be an important reservoir for H. pylori, with nearly 50% of the virulent
type that could possibly lead to gastroduodenal disease.
PMID: 29634191 [PubMed - in process]

Source: Southeast Asian Journal of Tropical Medicine and Public Health - April 14, 2018 Category: Tropical Medicine Tags: Southeast Asian J Trop Med Public Health Source Type: research