Multilocus sequence typing of brucella isolates from thailand.
MULTILOCUS SEQUENCE TYPING OF BRUCELLA ISOLATES FROM THAILAND.
Southeast Asian J Trop Med Public Health. 2016 Nov;47(6):1270-87
Authors: Chawjiraphan W, Sonthayanon P, Chanket P, Benjathummarak S, Kerdsin A, Kalambhaheti T
Abstract
Although brucellosis outbreaks in Thailand are rare, they cause abortions
and infertility in animals, resulting in significant economic loss. Because
Brucella spp display > 90% DNA homology, multilocus sequence typing (MLST)
was employed to categorize local Brucella isolates into sequence types (STs) and to
determine their genetic relatedness. Brucella samples were isolated from vaginal
secretion of cows and goats, and from blood cultures of infected individuals. Brucella
species were determined by multiplex PCR of eight loci, in addition to MLST
based on partial DNA sequences of nine house-keeping genes. MLST analysis
of 36 isolates revealed 78 distinct novel allele types and 34 novel STs, while two
isolates possessed the known ST8. Sequence alignments identified polymorphic
sites in each allele, ranging from 2-6%, while overall genetic diversity was 3.6%.
MLST analysis of the 36 Brucella isolates classified them into three species, namely,
B. melitensis, B. abortus and B. suis, in agreement with multiplex PCR results. Genetic
relatedness among ST members of B. melitensis and B. abortus determined
by eBURST program revealed ST2 as founder of B. abortus isolates and ST8 the
founder of B. melitensis isol...
Source: Southeast Asian Journal of Tropical Medicine and Public Health - Category: Tropical Medicine Tags: Southeast Asian J Trop Med Public Health Source Type: research