Efficient Detection of Environmental Estrogens Bisphenol A and Estradiol By Sensing System Based on AuNP-AuNP-UCNP Triple Structure

Publication date: April 2018 Source:Chinese Journal of Analytical Chemistry, Volume 46, Issue 4 Author(s): Qiao-Feng LI, Shu-Yue REN, Yu WANG, Jia-Lei BAI, Yuan PENG, Bao-An NING, Quan-Jun LYU, Zhi-Xian GAO A sensing system based on AuNP-AuNP-UCNP triple structure for efficient detection of dual targets bisphenol A and estradiol was constructed. In the preparation of triple structure, the gold nanoparticles (AuNPs) and upconversion nanoparticles (NaYF4: Yb, Er, Gd, UCNPs) were synthesized and surface modified. Then, the two nanoparticles and their aptamers were connected to form two kinds of optical fluorescent probes. A nucleic acid sequence that matches with two aptamers was designed, rendering the probes to get close based on the principle of complementary base pairing. On the basis of this, a sensing system with triple structure was prepared, and its connecting effect was characterized by TEM. With this system, dual targets of bisphenol A and estradiol were detected efficiently and conveniently through quantitatively determination by fluorescence and UV spectrophotometer. At the reaction temperature 30 °C and pH 7.8, this method exhibited good linear range for determination of bisphenol A and estradiol from 2 ng mL–1 to 200 ng mL–1 and from 10 ng mL–1 to 150 ng mL–1, with the limits of detection of 0.2 ng mL–1 and 0.5 ng mL–1, respectively. This sensing system with triple structure owned better specificity to structural and functional analogues, sho...
Source: Chinese Journal of Analytical Chemistry - Category: Chemistry Source Type: research