Effect Of Plasma Pen Treatment And Plasma Activated Medium (PAM) On Cancer And Normal Cells

Publication date: February 2018 Source:Clinical Plasma Medicine, Volume 9, Supplement Author(s): Dominika Sersenová, Helena Gbelcová, Adam Polakovič, Vanda Repiská, Zdenko Machala Non-thermal atmospheric pressure plasma has recently found an ever growing use in medicine; including development of new cancer treatments. The most significant factor, produced by plasma that influence cancer cells are reactive oxygen and nitrogen species (RONS). RONS react with the surrounding air, cellular aqueous media and with cells themselves; however, the exact mechanism of their interaction with the cells is not yet fully understood. Some of the studies suggest that plasma is able to induce apoptosis in cancer cells and has a potential to selectively kill cancer cells without causing a major destruction of normal cells [1]. Plasma can be applied both directly on cell or tissues or indirectly – by plasma-activated medium (PAM). It is a cellular medium, which was treated by plasma and then applied onto the cells, so the cells interact only with RONS produced in PAM [2]. The aim of this study was to test in vitro the effect of plasma on cancer cells A375 (human melanoma epithelial cells) and normal cells HEK293T (human embryonic kidney cells). As a medium we used DMEM with 10% FBS. The first part focuses on direct treatment of cells by our design of air corona plasma pen [3,4]. In the second part, we evaluated the effect of PAM on the cells. We used discharges generated in atmosph...
Source: Clinical Plasma Medicine - Category: Research Source Type: research