Sensitivity Of Murine Melanoma Cells B16 To Plasma Needle Treatment

Publication date: February 2018 Source:Clinical Plasma Medicine, Volume 9, Supplement Author(s): Jorge Humberto Serment Guerrero, Karina Giron Romero, Régulo López-Callejas, Rosendo Peña-Eguiluz Melanoma is the most dangerous form of skin cancer originated from the pigment-producing melanocytes in the basal layer of the epidermis. Its capability to produce metastasis, along with the development of quimio or radioresistance makes this kind of cancer very dangerous and hard to heal, so is important an early detection as well as the search of new treatments. B16 murine cell line has been used extensively as a model for the study of this type of cancer. Non thermal plasma needle has been effectively used in the inactivation of microorganisms, in the disinfection of several types of tissues and to accelerate the wound healing processes. It also has been tested as an inductor of apoptosis in HepG2 cancer cell line. The observed effects of plasma upon cells has been attributed to the generation of reactive oxygen/nitrogen species (RONS), which can disrupt the plasmatic membrane and react with several biomolecules including DNA. The aim of the present work is to evaluate the sensitivity of the murine melanoma cell line B16 to a helium-generated plasma needle exposure. B16 cells were grown at 37°C and 5% CO2 atmosphere, in minimal essential medium with 10% FBS. Cells were harvested by tripsinization, washed twice with Hanks balanced saline solution and further incubated for ...
Source: Clinical Plasma Medicine - Category: Research Source Type: research