Radiobiological Response Of Breast Cancer Cells, Exposed To Atmospheric Pressure Plasmas And Ionizing Radiation, An In-Vitro Essay. (Project Overview)

Publication date: February 2018 Source:Clinical Plasma Medicine, Volume 9, Supplement Author(s): A.G. Cordero, S. Quirós, M. Porras, E. Avendaño The purpose of this project is to describe the application of an ionized gas to different lines of breast cancer cells, added to application of a clinical photon beam, by measuring variations in cell death rate. This procedure may lead to implementation of a new in vitro technique for studying new radiobiological approaches for adjuvant methods to radiotherapy techniques. Characterization of the plasma generator An in-house developed Cold Atmospheric Plasma "CAP" will be evaluated in terms of its functionality and features in geometry and operation. The characteristics of the plasma generated using an Argon-Helium gas mixture, should be analyzed regarding its possibilities of interaction with a biological medium, depending on properties such as the Power of the source; Gas pressure; Visible and infrared emission wavelength by FTIR Microscopy and Ultra-Violet (UVA, UVB, UVC) by UV absorption spectroscopy [1]; Gas temperature; Exposure time; Determination of reactive species, and Gas flow (5 l/min). Biological Evaluations The cell lines of human breast adenocarcinoma (MCF-7, MDA-MB-231, MDA-MB-468, Hs 578T, T-47D, MDA-N and BT-54), will be placed in plates of deposits with regular flat bottom for the application of ionizing radiation and CAP, using a cell culture medium; RPMI 1640 supplemented with 10% fetal bovine serum, 100...
Source: Clinical Plasma Medicine - Category: Research Source Type: research