Air Dielectric Barrier Discharge Plasma Source For In Vitro Cancer Studies

Publication date: February 2018 Source:Clinical Plasma Medicine, Volume 9, Supplement Author(s): Ioana Cristina Gerber, Cosmin Teodor Mihai, Lucian Gorgan, Mitica Ciorpac, Alexandru Nita, Valentin Pohoata, Ilarion Mihaila, Ionut Topala For most cancer studies involving plasma treatments, the cell cultures require a significant amount of manipulation before and after treatment. This increases the risk of contamination and of damaging the cells. The well plates are used for growing cell cultures, and a discharge adapted to their geometry would minimize cell culture manipulation during exposures and biological assays. Our laboratory developed an air dielectric barrier discharge (DBD) plasma inside a well plate focusing on treating an entire well plate column at a time.[1] The discharge, presented in Figure 1, was generated using an AC neon transformer (sinusoidal high voltage waveform, 15 kV maximum peak-to-peak output voltage, 50 Hz frequency). For a 96 well plate, the following electrode assembly was designed: a planar electrode was placed under the well plate, and 8 stainless steel cylindrical electrodes were inserted in the wells containing the cells. The geometry of the system provides a volume discharge inside the wells, preventing power losses in the surrounding areas. The well plates act as a dielectric layer for the DBD, while also shielding the cells from any potential damages caused by external factors. Figure 1. Air DBD plasma generated inside the well pl...
Source: Clinical Plasma Medicine - Category: Research Source Type: research