Emergence of co-carbapenemase genes, bla(oxa23), bla(vim) and bla(ndm) in carbapenemresistant acinetobacter baumannii clinical isolates.
This study investigated presence of carbapenemase genes among
carbapenem-resistant Acinetobacter baumannii (CRAB) clinical isolates and their
clonal relationships. Fifty-six CRAB isolates were collected from patients admitted
to Hua Hin Hospital, Prachuap Khiri Khan, Thailand. PCR amplification and
DNA sequencing were used to identify blaOXA23, blaOXA40, blaOXA58, blaVIM, blaSIM and
blaNDM. Clonal relationship was explored using repetitive element palindromic
(REP)-PCR. Plasmid profiling was obtained from EcoRI-digested fragments. The
CRAB isolates were classified by REP-PCR into 12 groups, with 71% belonging
to group I, which was associated with the presence of blaOXA23. Co-existence of
blaOXA23 + blaVIM2 (n = 20), blaOXA23 + blaNDM1 (n = 2), blaVIM2 + blaNDM1 (n = 1), and
blaOXA23 + blaVIM2 + blaNDM1 (n = 1) were discovered. The emergence of CRAB carrying
multiple types of carbapenemase genes (the first such report in Thailand)
is a worrying phenomenon and public health measures should be put in place
to prevent any serious nosocomial infection and to contain the spread of such
CRAB genotypes.
PMID: 29620807 [PubMed - in process]
Source: Southeast Asian Journal of Tropical Medicine and Public Health - Category: Tropical Medicine Tags: Southeast Asian J Trop Med Public Health Source Type: research
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