High level of shiga toxin-producing escherichia coli and occurrence of stx-negative e. coli o157 from raw meats: characterization of virulence profile and genetic relatedness.

HIGH LEVEL OF SHIGA TOXIN-PRODUCING ESCHERICHIA COLI AND OCCURRENCE OF STX-NEGATIVE E. COLI O157 FROM RAW MEATS: CHARACTERIZATION OF VIRULENCE PROFILE AND GENETIC RELATEDNESS. Southeast Asian J Trop Med Public Health. 2016 Sep;47(5):1008-19 Authors: Sirikaew S, Sukkua K, Rattanachuay P, Khianngam S, Sukhumungoon P Abstract Raw meats, especially beef, are particularly prone to Shiga toxinproducing Escherichia coli (STEC)/enterohemorrhagic E. coli (EHEC) contamination. However, data regarding their quantity in raw meats are seldom reported in Thailand. Among four common meat types, beef possessed highest value of stx1-producing E. coli (STEC1) contamination in February 2015 [> 1,100 most probable number (MPN)/g] and stx2-producing E. coli (STEC2) highest MPN/g (460) in March of the same year. STEC2 was found, for the first time, in shrimp samples in March and April, 2015 with MPN/g value of 6.6 and 9.3, respectively. EHEC at 3 MPN/g was detected in only one (2%) beef sample. Even though stx-negative E. coli O157 from beef has rarely been reported in Thailand, isolation of E. coli O157 using immunomagnetic separation method revealed that four strains (PSVX-1, PSVX-2, PSVX-3, and PSVX-4) from three (8%) beef samples were shown to be stx-negative E. coli O157. These strains were members of phylogenetic group A and were multi-drug resistant. Genetic relatedness as determined by polytrinucleotide (GTG)5-PCR and BOX-PCR showed identical D...
Source: Southeast Asian Journal of Tropical Medicine and Public Health - Category: Tropical Medicine Tags: Southeast Asian J Trop Med Public Health Source Type: research