Effects of Pseudomonas aeruginosa infection on the expression profile of transient receptor potential-like (TRPL) gene from Drosophila melanogaster.
Effects of Pseudomonas aeruginosa infection on the expression profile of transient receptor potential-like (TRPL) gene from Drosophila melanogaster.
Roum Arch Microbiol Immunol. 2016 Jan-Jun;75(1-2):32-6
Authors: Mihalache (Radu) MR, Raþiu AC, Neagu A, Lazãr V, Chifiriuc MC, Ecovoiu AA
Abstract
In an endeavor to monitor the effects of
experimental infections with Pseudomonas
aeruginosa on gene expression profiles of the
eukaryotic model host, we focused on transient
receptor potential-like (trpl) pleiotropic gene of
Drosophila melanogaster (the fruit fly). Herein we
report qRT-PCR data derived from experimental
infections of male fruit flies with different genetic
backgrounds by pricking and ingesta procedures, as
compared to previous results obtained by microarray
technology consecutive to ingesta experiments.
Whenever statistically significant, the qRT-PCR
results obtained for the whole body and intestine of
Oregon wild-type flies infected with P. aeruginosa
are in agreement with the microarray ones. Both
expression profiling technologies revealed similar
values of downregulation, supporting the robustness
of trpl as a novel gene model for inquiring hostpathogen
interactions. On the other hand, the
downregulation of trpl in gammaCop mutant males
is not confirmed by qRT-PCR data, suggesting that
this mutant background is more sensitive to
environmental and experimental conditions.
PMID: 29616531 [PubMed - in process]
Source: Roumanian Archives of Microbiology and Immunology - Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
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